DropletONE

Integrated Droplet Generation, Analysis & Sorting System

DropletONE is a fully integrated platform that streamlines droplet generation, intelligent analysis, and precise sorting – all within a single device. Designed for efficiency and ease of use, it enables users to produce highly consistent, monodisperse droplets in minutes using minimal sample input and straightforward parameter settings.

Product Overview

DropletONE integrates droplet generation, quality control, sorting, and analysis in a single platform. The system operates entirely label-free, relying solely on advanced imaging to eliminate the need for staining. Its built-in feedback loop allows real-time control and manipulation of droplet size, ensuring optimal performance across a wide range of applications. This feedback mechanism can be driven by imaging alone or combined with flow sensors, enabling advanced control while reducing potential microfluidic failure points.

At the core of DropletONE is its Multidimensional Cell Analysis capability, which surpasses traditional methods by providing real-time insights into droplet production. This empowers operators to observe and adjust the process dynamically, responding immediately to changing conditions. The system’s holistic analytical approach uncovers complex cellular interactions and behaviors, playing a crucial role in advancing targeted therapies and deepening our understanding of dynamic biological systems.

DropletONE supports multi-parameter sorting of droplets containing diverse targets like cells, spheroids, microorganisms, algae, and other particles. Its ultra-high frame rate imaging, enabeled by the built-in ODIN sensor, captures critical parameters such as morphology, size, velocity, and position with exceptional sensitivity, significantly enhancing data quality and experimental throughput.

With constant flow control for uniform droplet size, DropletONE is ideally suited for high-throughput, high-precision experiments such as cell-cell interaction studies, drug efficacy testing, and enzyme activity assays, making it an indispensable tool in life science and biomedical research.

DropletONE has some major advantages over other droplet generators and can be used for a large range of applications. Key Benefits include:

Real-Time Droplet Control	Precisely manage droplet size, spacing, and generation rate with dynamic feedback mechanisms that work in combination with or even without flow sensors.
Open Compatibility	No vendor lock-in – supports a wide range of droplet generation chips and Raydrop droplet generators.
Advanced Imaging & Analysis	Real-time brightfield imaging enables detailed, label-free analysis of droplet contents, including encapsulation status, quantity, and particle type.
Efficient Sorting	Quickly count, measure, and filter to identify and isolate desired target populations with precision.
Versatile Applications	Supports generation of droplets, double emulsions, microbubbles, PLGA, and LNPs.

Droplet Generation	Precise generation and control of droplets via feedback-loop
Flow Control	Adjustable flow rates ranging from µL/min to mL/min, offering flexible options for various experimental needs.
Droplet Parameters	Supports droplet diameters from tens to hundreds of micrometers, with tunable size and generation frequency.
Imaging Sensor	High-performance camera with integrated analysis and feature extraction
Frame Rate	210 fps @ 1280×1024 px, up to 10000 fps @ 400×32 px, monochrome, 10bit color depth
Sensitivity	Wavelength range 300-1000 nm, 58% QE @ 550nm peak ; 62% QE @ 630nm; global shutter
Readout Regions	2 freely selectable, simultaneous readout regions, e.g. for two stage processes, immediate quality control or parallelization.
Latency	Image capture and analysis latency is under 200 microseconds, enabling real-time responsiveness.
Illumination Unit	Single-color pulsed illumination (0.1–5 µs flash duration) delivers ultra-sharp imaging, eliminating motion blur and minimizing photodegradation in sensitive samples.
Software	User-friendly and feature-rich graphical user-interface for observation, analysis, and control
Analysis	Real-time analysis of various detection parameters like size, velocity, position, granularity, circumference etc.
Gating	Freely configurable gating; graphical marking in scatter plot; arbitrary points polygon
Triggers	Freely programmable and independent triggers; latency down to 200µs (frame rate dependent)
Storage Format	RAW; AVI uncompressed; MJPEG; MP4; XLS; XLSX; CSV (infinite recording)
Size & Weight	Compact all-in-one system that easily fits into every lab
Dimensions (L×W×H/mm)	428×380×322
Weight	25 kg

Download the full DropletONE Specification

Sorting Method	Active Dielectrophoretic
Sorting Efficiency, Selectivity, Purity	Up to 99 % demonstrated
Droplet Sizes	10 µm to 1 mm diameter
Detection Methods	Bright field image
Detection Parameters	Brightness/intensity; brightness contrast; circumference; area; surface roughness; colocalization; particle count; area contrast; fill factor; elongation; circularity; size; velocity; position; granularity; removal of small dirt particles; area of no contrast; contrast within particles; particles touching walls; particles touching image boundaries; particle position; particle velocity
Quality Control	In-line, multispectral image per particle pre & post sorting; all droplets recorded
Sample Viability	Further downstream usage of the sample; maximized cell viability
Assay-based Sorting	Yes

Download the full DropletONE Specification

Droplet Generation, Manipulation, Analysis & Sorting

(Singel) Cell Sorting

(Single) Cell Analysis

Cell Therapy

Cell Line Development

Drug Discovery

Antibody Discovery

Drop-Seq

(RNA / DNA) Sequencing

Particle Synthesis, Analysis & Sorting

Bacteria Analysis & Sorting

Algae Analysis & Sorting

Yeast Analysis & Sorting

Sperm Analysis & Sorting

Organoid Generation, Screening & Sorting

DropletONE is available in the following variants:

DGO200	Integrated droplet generation and imaging system featuring dual precision pressure controllers.
DGO300	Integrated droplet generation and imaging system featuring triple pressure regulation for enhanced microfluidics.
DGO200 Sort	Integrated droplet generation, imaging and sorting system featuring dual precision pressure controllers.
DGO300 Sort	Integrated droplet generation, imaging and sorting system featuring triple pressure regulation for enhanced microfluidics.

The following consumables are available for DropletONE:

Microfluidic Chip DGO30	Water-in-oil droplet generation chip (COC), featuring dual inlets and a single outlet (channel w/d 30×30μm).
Microfluidic Chip DGO50	Water-in-oil droplet generation chip (COC), featuring dual inlets and a single outlet (channel w/d 50×30μm).
Microfluidic Chip DGO100	Water-in-oil droplet generation chip (COC), featuring dual inlets and a single outlet (channel w/d 100×60μm).
Microfluidic Chip DGO150	Water-in-oil droplet generation chip (COC), featuring dual inlets and a single outlet (channel w/d 150×60μm).
SCO50	Double-emulsion chip designed for single cell encapsulation. Available with two different surface treatments.

DropletONE Product Catalogue

DropletONE Specification

Droplet Generation

Easily generate stable, uniform droplets by means of a fully automated feedback loop that controls droplet size, spacing, and frequency. The system operates smoothly with or without flow sensors, ensuring a seamless user experience.

Droplet Content

Differentiate and classify droplet contents using advanced imaging technology. Effortlessly analyze complex assays with image-linked data points, each one directly accessible as a one-click-solution in the intuitive software interface.

Droplet Analysis

Real-time brightfield imaging enables sophisticated, non-invasive, and gentle analysis of droplets and their contents. Additionally, the fully integrated online data analysis supports continuous in-line quality control.

Droplet Sorting

Easily identify, analyze and sort desired target populations at high speed and outstanding accuracy. The intuitive software interface enables effortless, visual gating directly within the scatter plot.

Product Catalogue

Cell & Gene Therapy

DropletONE supports the development of advanced therapies by enabling precise cell handling, viability analysis, and accelerated development workflows. Applications include:

Cell Therapy: DropletONE enables rapid isolation of functional immune cells and detailed viability assessments, ensuring greater consistency and quality in therapeutic processes.
Cell Line Development: The system enhances selection efficiency and clonality, speeds up screening, and improves overall cell viability – making it a powerful tool for robust cell line development.

Genomics & Molecular Biology

From single-cell sequencing to synthetic biology, DropletONE delivers unmatched throughput, accuracy, and control for complex molecular workflows. Explore the following applications, among many others:

RNA / DNA Sequencing & Single-Cell Genomics: Perfect for Drop-seq and similar applications, DropletONE boosts droplet generation containing useful populations by 100×, encapsulating droplets 20× faster, and reduces error rates from 5% to just 0.1%, with real-time quality control and minimal interference from cell-free RNA signals.
Synthetic Biology and Genomics: Enables high-throughput screening of gene mutants and functional droplets, playing a key role in optimizing metabolic pathways and advancing synthetic biology research.
Basic Life Science Research: Facilitates real-time, quantitative analysis of microscopic processes such as cell signaling and cell-cell interactions, driving deeper biological insights.

Drug Discovery & Functional Screening

DropletONE streamlines discovery and screening workflows with high-throughput capabilities, kinetic analysis, and real-time monitoring of biological interactions.

Antibody Discovery and Screening: Supports the discovery, optimization, and high-throughput screening of B cells, while enabling detailed analysis of antibody-antigen interactions.
Drug Discovery & Bioactivity Assays: Offers droplet-based kinetic analysis and sorting for precise evaluation of enzyme activity, antibody affinity, and drug-cell interactions.
Organoid Generation & Online Screening: Facilitates the generation of organoids and enables real-time, high-throughput screening, advancing research in 3D cell culture systems.

Microbiology, Safety & Materials

DropletONE is a robust solution for microbial analysis, environmental monitoring, and precision particle synthesis.

Microbiology: Enables high-throughput characterization and sorting of microbial species, supporting growth assays and phenotypic profiling.
Environmental Monitoring & Food Safety: Allows rapid pre-screening and accurate sorting of pathogenic microorganisms in water and food samples, supporting safety and regulatory compliance.
Particle Synthesis: Delivers precise droplet generation, manipulation, and sorting, which is ideal for producing uniform particles or emulsions with tight size control.

Take control of your experiments with DropletONEs intuitive user interface, designed to streamline workflows, boost productivity, and accelerate your path to discovery.

NAll-in-One Droplet Solution: Integrates droplet generation, quality control, sorting, and analysis in a single platform.

NProven Performance: Built on years of industry experience to deliver stable, highly repeatable, and low-dispersion droplet generation.

NEnhanced Encapsulation: Comprehensive droplet solutions significantly boost encapsulation efficiency and efficacy, and reduce error rates.

N Intelligent Software: User-friendly interface with intuitive operation powered by smart algorithms delivers over 30 object parameters such as size, velocity, position, and contrast.

N Instant Quality Control: Fully integrated real-time data analysis eliminates the need for post-processing – even at droplet rates above 2000 droplets per second.

N Flexible Integration: Customizable trigger signal length and duration ensures compatibility with various sorting systems and flow rates.

N Comprehensive Imaging: Count, measure, and filter effortlessly to identify and separate the high performers. Equipped with an X-Y-Z precision stage and CMOS camera for accurate real-time imaging, while reducing data storage needs by up to 95%.

N High Repeatability: Ensures high cell viability and maintains sterility with enclosed channels. Exceptional flow control ensures consistent droplet size and low polydispersity.

NEasy Connectivity: Simple and stable chip connections streamline the setup and improve experimental efficiency.

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