New method to enhance Drop-Seq using Sensific’s ODIN system
Patricia Schwilling showcased an advanced method to enhance Drop-Seq using Sensific’s ODIN system at SPIE BiOS 2024, published in SPIE Proceedings.
Originally introduced in 2015, Drop-Seq revolutionized single-cell RNA sequencing by offering an open-source, cost-effective, and high-throughput analysis of gene transcripts. This technique involves encapsulating a diluted suspension of cells and barcoded beads in water-in-oil droplets, aiming for one cell and one bead per droplet. However, erroneous encapsulations of multiple cells or beads reduce efficiency and increase costs.
Our innovative approach leverages ODIN and one of our dielectrophoretic (DEP) sorting microchannel to detect and eliminate these errors. This allows for a higher concentration of beads, significantly boosting the number of successful encapsulations (one bead + one cell) while minimizing errors.
Reference: P. Schwilling, J. Pfeil, M. Güttler, O. Marti, “Increasing efficiency, throughput and purity of Drop-seq through detection and sorting of correctly configured droplets,” Proc. SPIE 12853, High-Speed Biomedical Imaging and Spectroscopy IX, 128530B (12 March 2024)